The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Dicyclomine and Tramadol Hydrochloride in bulk and in combined dosage form. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Dicyclomine and Tramadol Hydrochloride show an isoabsorptive point at 222 nm in 0.1 N NaOH. The second wavelength used is 276 nm, which is the λ-max of Tramadol Hydrochloride in 0.1 N NaOH. The linearity was obtained in the concentration range of 2.5-15 μg/ml for Dicyclomine and 12.5-75 μg/ml Tramadol Hydrochloride. The concentrations of the drugs were determined by using ratio of absorbance at isoabsorptive point and at the λ-max of Tramadol Hydrochloride. The method was successfully applied to pharmaceutical dosage form because of no interference. The results of analysis have been validated by recovery studies.
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